highQu PCRbeam™ Fast PCR Detection Kit is a convenient tool for fast detection of gene-specific amplification products obtained by PCR, LAMP or RPA. The detection is based on immunological reaction driven by Biotin and FITC (fluorescein isothiocyanate), thus the amplified DNA shall include Biotin and FITC labels. The PCR amplification has to be performed with one primer labeled with FITC at 5’-end and one primer labeled with Biotin at 5’-end. Alternatively the use of one of the labeled primers can be replaced by gene-specific FITC or Biotin labeled probe. Kit includes PCRbeam™ Membrane Strips that are coated with biotin-ligand on the test band and an anti-rabbit antibody on the control band. The bottom part of the strip which is used for sample application contains an anti-FITC antibody attached to gold particles. PCRbeam™ Detection Buffer is Tris-buffered saline enabling the detection. The PCRbeam™ Fast PCR Detection Kit can be applied for established tests or home-brew assays as a fast and sensitive yes/no detection method. The detection sensitivity is up to 100 fold higher than the one achievable with ethidium bromide stained gels what provides an environment friendly save and economical alternative to the use of mutagen stains. For establishing sensitive PCR-based tests before PCRbeam™ detection we recommend the use of hot-start PCR enzymes or master mixes, like highQu ALLin™ Hot Start Taq Mastermix or ALLin™ Hot Start Taq DNA Polymerase. Principle: The membrane strip is soaked for 10 minutes into the vial with the detection buffer mixed with PCR product. The lateral sample flow driven by gold particles moves the solution up the strip. FITC labeled DNA strand binds with the anti-FITC antibody on the gold particle and Biotin labeled DNA strand is caught by Biotin ligand attached to the test band. As both DNA strands remain hybridized at room temperature, the test band builds an aggregate that develops red-blue color. Excess gold particles that were not caught by FITC move up the strip and the anti-FITC antibody binds to the anti-rabbit antibody to develop the red-blue colored control band. If there is no PCR product in the reaction, then only the control band will be visible. If there is a specific product, the test band will be colored as well.

highQu Synthetic Carrier RNA is designed to be used in all kind nucleic acid purification and precipitation procedures as a carrier and co-precipitant of nucleic acids. It is especially useful to increase the amount of RNA or DNA pellet in low concentrated solutions, in such procedures, as viral RNA extraction from human specimen samples. In contrast to commonly used carrier RNAs such as tRNA, yeast RNA, or sonicated salmon sperm DNA, the Synthetic Carrier RNA is free from animal or yeast RNA contamination. Coprecipitated RNA and DNA can be directly used for all common downstream applications, such as PCR or RT-PCR, as well as highly sensitive qPCR. The use of carrier RNAs for coprecipitation of nucleic acids may interfere with spectrophotometrical concentration measurements. The presence of carrier RNAs in the RNA or DNA solution may have some influence on certain enzymatic reactions performed by such enzymes that act on all nucleic acid molecules, for example T4 Polynucleotide Kinase or Terminal DNA Transferase.

highQu PCR Water is a supplementary high quality reagent for all demanding PCR and qPCR and other molecular biology applications. It saves time being on your bench and guaranties the purity of reactions and inhibition-free performance of PCR reagents. highQu PCR Water is a deionized, membrane filtered water continuously tested in ultrasensitive qPCR and PCR applications, in amplification of long targets and highly specific detection of few copies of templates.

Proteinase K (Molecular Biology Grade Solution) is a serine peptidase with a very high specific activity and a broad spectrum of protein digestion possibilities. The solution is designed to be used for protein degradation (up to tetrapeptides) during the cell lysis and RNA/DNA extraction procedures under hush reaction conditions such as the higher temperatures and the presence of detergents. The enzyme efficiently degrades DNases and RNases during nucleic acid isolation process. The high purity of the Proteinase K and controlled absence of both DNAse and RNase contamination ensures the integrity of nucleic acids. The 100% enzyme activity (when stored at -20°C) is guaranteed for at least two years after production. However, the experiments proved that the proteinase remains close to 90% active even when stored at +37°C for 18 months. The enzyme is supplied as a 20 mg/ml concentrated solution with an average specific activity of more than 800 u/ml. Active in all common buffers used for cell lysis and RNA/DNA extraction, in a presence of urea, SDS and guanidinium salts Stable at high temperature of up to 56°C Can be inactivated by heating at 65oC for 20 minutes or at 75oC for 10 minutes Active in a pH range of 4–12 with an optimum pH 7.5–8.0 The Proteinase K gene from album expressed in yeast host. The quality limit of allowed host DNA presence is ≤ 0.25 pg/U measured by qPCR what is about a half when compared to other suppliers. Unit Definition Folin & Ciocalteu’s method - One unit is required to hydrolyze urea-denaturated hemoglobin producing color equivalent of 1 μmol tyrosine in 1 minute at 37°C and pH 7.5, 1 U = 1 m Anson U.

highQu ready to use Prestained Protein Ladders are mixtures of highly recombinant proteins with coupled chromophores providing sharp protein bands and bright colors on denaturing polyacrylamide gels. The high ladder purity allows for exceptional stability and room short term temperature storage. Ladders are ready to be directly loaded on gels without any preparation or heating. They provide sharp bands for approximate protein sizing and allow for monitoring of electrophoresis process and Western transfer efficiency.

StainIN™ GREEN Nucleic Acid Stain is a significantly safer alternative to ethidium bromide. It is same easy to use, 4X as sensitive (under Blue LED) and much more secure. More economical as other green dyes, this stain can be also used and disposed with less environmental and health concerns compared to ethidium bromide. It is a fluorescent dye that allows detection of >0,1 ng of DNA in both agarose and polyacrylamide gels. It binds to both dsDNA, ssDNA and RNA and emits green fluorescence when bound to DNA and red fluorescence when bound to RNA detectable under the UV or Blue light and documented with same filters as similar green dyes. StainIN™ GREEN is ideal for DNA extraction from gels for cloning. Smaller than ethidium bromide carcinogenicity has been proved by Ames-test. Mammalian cell mutagenicity tests, both mouse marrow erythrocyte micronucleus and spermatocyte chromosomal aberration tests gave negative mutagenicity results. Carcinogenicity and toxicity of the widely used Ethidium Bromide (EtBr) is one of the biggest safety and environmental problems in the labs where gel electrophoresis is a daily routine. The less toxic nucleic acid dyes address this problem and minimize concerns. The StainIN™ GREEN (SYBR Green-like stains alternative), (SYBR® Green is a registered trade mark of Molecular Probes Inc.) and StainIN™ eco-RED Nucleic Acid Stains by highQu provide a much less-carcinogenic, significantly safer alternative to EtBr while delivering even several times higher detection sensitivity. Same like EtBr, these stains are used during the process of electrophoresis in an agarose gel or PAAG and in the electrophoresis running buffer. Nucleic acid stains are typically divided into two groups – they are either intercalating dyes or minor groove binders. The StainIN™ GREEN and StainIN™ eco-RED Nucleic Acid Stains belong to the group of intercalating dyes that bind to NA and produce the fluorescence signal. Same like many other suppliers of similar products, highQu retains the right to keep the precise chemical information undisclosed and proprietary. The StainIN™ GREEN Nucleic Acid Stain as well as the StainIN™ eco-RED Nucleic Acid Stain are both supplied in water. Detailed safety information is available in MSDS sheets of the stains. Despite proven less mutagenicity, all nucleic acid stains, including SYBR® Green or other shall be handled with care. Used electrophoresis buffer with a stain as well as melted gels shall be run through approved (carbon) filters. If the absence of residual fluorescence is confirmed, the liquids can be disposed with plenty of water down the drain. Alternatively, all used liquids can be autoclaved as the StainIN™ GREEN and StainIN™ eco-RED Nucleic Acid Stains degrade during the long exposure to above 100ºC temperature. The safety office shall be consulted periodically to match local regulations, as they vary and change.