Reliable and robust Thermophilic DNA Polymerases for Standard PCR & demanding PCR applications
Selection of enzymes and master mixes for the most demanding PCR applications
|Fast cycling||GC/AT rich PCR||Max product||High Yields||Direct PCR||Multiplex PCR||Direct gel loading|
|Fast, direct crude sample PCR, includes ALLin™ HS Taq DNA Polymerase in 2X mastermix with red loading dye (available separately)||●||●||●||5 kb||●||●●●||●||●|
|Robust Taq with ALLin™ buffer for standard, fast, GC rich PCR||●||●||●||6 kb||●●||●||●|
|ALLin™ Taq DNA Polymerase in 2X mastermix with loading dye||●||●||●||6 kb||●●||●||●||●|
|ALLin™ Taq DNA Polymerase in 2X mastermix||●||●||●||6 kb||●●||●||●|
|Classical Taq, magnesium supplied separately||5 kb||●|
The most used DNA Polymerase for PCR
PCR, or a Polymerase Chain Reaction, is a well-established DNA amplification technique used to amplify a specific segment of template DNA. Since many years, it remains a crucial tool in scientific research applications, medical diagnostics, genetic research, analysis of genetically modified food, and forensic science.
How does the PCR work?
The PCR works by utilizing cycles of heating (DNA denaturation) and cooling (primer annealing to the complementary DNA sequences) to replicate a specific DNA segment by the DNA polymerase which is active at high temperatures. This process involves the use of a DNA template, short template-specific oligonucleotide primers, nucleotides as building blocks or substrate, and an enzyme, a thermophilic polymerase.
Thermophilic DNA Polymerases
The thermophilic DNA Polymerases are the main tools of the PCR reaction. The most known polymerase is the Taq DNA Polymerase, a recombinant enzyme originally derived from Thermophilus aquaticus bacteria. Though numerous new polymerases have been discovered, cloned, sequenced, mutagenized and developed, Taq DNA Polymerase still remains the most crucial tool in daily laboratory testing applications, as well as in sophisticated diagnostic kits.