OEM REAGENTS FOR KIT DEVELOPERS

Why to choose highQu molecular biology reagents? 

• Professionally simple - easy-to-use reagents, fast protocols
• Scientific track record, growing number of publications since 2013
• Recognized as partner with unique add-on services

• Download highQu Flyers

--------------------------------------------------------------------

NEW: Direct qPCR and RT-qPCR Mixes - Lyo-Ready and Air-Dryable versions available

for qPCR applications with crude samples or inhibitor-rich templates

SampleIN™ qPCR Mixes exhibit exceptional tolerance to various PCR inhibitors and enable efficient amplifications from crude samples.

SampleIN™ Mixes Tolerate common inhibitors, such as:

• 5-7% crude saliva and crude blood in the reaction
• Chemicals left after NA extractions (guanidine, alcohols, SDS) 
• Blood compounds (hematin, hemoglobin, hemin, immunoglobulins)
• Saliva and urine compounds (urea)
• Plant, soil samples (chlorophyl, humic, tannic acids, quercetin cellulose) 

 SampleIN™ Collection:

• DNA templates: SampleIN™ Direct qPCR Probe Mix and SampleIN™ Direct qPCR Probe ROX L Mix.

• For Kit Developers: DNA templates: Lyo-Ready SampleIN™ Direct qPCR Probe Mix (OEM upon request) for dry & ambient temperature stable assays.

• RNA & DNA templates: SampleIN™ 1Step RT qPCR Probe Mix and SampleIN™ 1Step RT qPCR Probe ROX L Mix.

• For Kit Developers: RNA & DNA templates: Lyo-ready SampleIN™ 1Step RT qPCR Probe Kit (OEM upon request) for dry & ambient temperature stable assays.

• For Kit Developers: RNA & DNA templates: AirDry SampleIN™ 1Step RT qPCR Probe Mix (OEM upon request) for dry & ambient temperature stable assays.

• ROX solution for high ROX mixes: ROX for qPCR Mixes, 50 µM

• Crude sample preparation: SampleIN™ Lysis Set for PCR/qPCR

Long-term and short-term stability at different temperatures of inhibitor-resistant SampleIN™ mixes in conventional, lyo-ready, and air-dryable formats:

--------------------------------------------------------------------

Tools for LAMP and other isothermal amplification-based assays

ALLin™ Bst DNA Polymerase

• Glycerol-free formulation of robust Bst DNA Polymerase, supplied with all-included buffer with dNTPs and proprietary reaction enhancer

ALLin™ Hot Start Bst Polymerase

• Glycerol-free formulation of robust Hot Start Bst Polymerase, supplied with all-included buffer with dNTPs and proprietary reaction enhancer
• The enzyme is blocked at ambient temperature due to molecular inhibition based hot-start technology, what is excellent for ambient reaction setup

ALLin™ HS Isothermal DNA Amplification Kit

• Hot Start Bst for an ambient temperature setup and minimized background
• Efficient 20 minutes DNA amplification at constant 55-70°C temperature
• ALLin™ format, supplied with water and a dye for fast real-time detection

ALLin™ HS Iso-Colorimetric DNA Amplification Kit

• Point of care and field colorimetric DNA target detection
• Point of care colorimetric LAMP - loop-mediated amplification with real-time monitoring

ALLin™ Isothermal DNA Amplification Kit

ALLin™ Isothermal 1Step RNA Amplification Kit

phi29 DNA Polymerase

--------------------------------------------------------------------

AirDry 4X 1Step RT qPCR Probe Mix

• Create room temperature stable assays faster, without the lyophilization equipment

--------------------------------------------------------------------

LYO-READY PCR /qPCR kits and enzymes

• Lyo-Ready 4X 1Step RT qPCR Probe Kit 
• Lyo-ready ORA™ qPCR Probe Mix, 4X
• Lyo-Ready Hot Start Taq Polymerase, 250 u/μl

--------------------------------------------------------------------

LYO-READY 1 Step RT-qPCR Kits for your RT-qPCR-based  assays

• QOP070LR Lyo-Ready 4X 1Step RT qPCR Probe Kit available as 2000/10000/100000 reactions
• Component1 (available separately) - glycerol-free, lyophilization-ready formulation of 4X qPCR mix for high sample volume input
• Component2 (available separately) - concentrated blend of Reverse transcriptase and RNase Inhibitor for lyophilization workflows
• Detects <5 RNA copies, tested for Sars-CoV-2 detection
• Ideal for multiplex reactions, for direct RT-qPCR without RNA extraction

--------------------------------------------------------------------

RT qPCR Kits, available in 5 / 50 / 500 ml scale

• Robust – inhibitor-resistant, work under a broad range of reaction conditions
• Fast - designed for fast-cycling protocols, which enables fast test results
• Outperforming – great results on complex templates, and in multiplexing
• Tested for Sars-CoV-2 detection. Check Application Note
• 1Step RT qPCR kits include either 4X or 2X qPCR master mix and a blend of thermostable HighScriber™ Reverse Transcriptase with RNase Inhibitor
• Kits can be supplied with PCR Water, kit components can be supplied separately
• RT qPCR Kit versions with low or high ROX concentration are available:
• #QOP070LR Lyo-Ready 4X 1Step RT qPCR Probe Kit
• #QOP070LT Lyo-Test 4X 1Step RT qPCR Probe Kit
• #QOP04 – 4X 1Step RT qPCR Probe Kit
• #QOP11 – 2X 1Step RT qPCR Probe Kit (w 2X qPCR mix)
• #WAT01 – PCR Water, a component of all the above-mentioned kits

--------------------------------------------------------------------

LYO-READY qPCR Mixes for your qPCR-based assays

• QPP070LR Lyo-ready ORA™ qPCR Probe Mix, 4X, available as 2000/10000/100000 reactions
• Glycerol-free, lyophilization-ready formulation of robust 4X qPCR mix for high sample volume input
• Universal mix - both standard and fast cycling, all probe qPCR assays, GC or AT rich templates
• Excellent for multiplexing and high throughput

--------------------------------------------------------------------

LYO-READY Hot Start PCR Enzyme for your PCR-based assays

• HSE010LR Lyo-Ready Hot Start Taq Polymerase, 250 u/μl
• Highly concentrated Taq Polymerase supplied with Lyo-Ready HS Protein Blend
• Optimized for freeze-dry process ensuring minimal glycerol and detergent concentration in lyophilization mixtures
• Special Protein Blend enables the hot start function of Taq Polymerase for low copy number target detection

--------------------------------------------------------------------

dNTPs available in 1 ml, 25 ml, 100 ml, and larger scale

• ≥99% HPLC-confirmed purity
• Pure from contaminating & deaminated nucleotides, pyrophosphates
• Free from nucleases & proteases, from bacterial & human DNA
• Stable during storage/usage & multiple freezing/thawing
• Contact us at info@highQu.com for Quotes and more information
• #NUM25B a 25 mM dNTP Mix of all 4 dNTPs in 1 ml, 25 ml, 100 ml and liter scale (PI example)
• #NUCA dATP, 100 mM Sodium Salt Solution, in 1 ml, 25 ml, 100 ml and liter scale
• #NUCC dCTP, 100 mM Sodium Salt Solution, in 1 ml, 25 ml, 100 ml and liter scale
• #NUCG dGTP, 100 mM Sodium Salt Solution, in 1 ml, 25 ml, 100 ml and liter scale
• #NUCT dTTP, 100 mM Sodium Salt Solution, in 1 ml, 25 ml, 100 ml and liter scale
• #NUCU dUTP, 100 mM Sodium Salt Solution, in 1 ml, 25 ml, 100 ml and liter scale

--------------------------------------------------------------------

LYO-READY Reverse Transcriptase and RNAse Inhibitor for your RNA-detection-based assays

• Lyo-ready RT7 Mix, the Reverse transcriptase and RNI blend available separately or in QOP070LR Lyo-Ready 4X 1Step RT qPCR Probe Kit
• HighScriber™ Reverse Transcriptase for the cDNA synthesis from complex templates at up to 55°C (available separately, or in a mix with RNase inhibitor)
• SecurRIN™ Advanced RNase Inhibitor available in big volumes for an efficient RNAse inhibition up to 60°C
• Flexible volumes of RT/inhibitor enzyme blends, for example #RTM03 - RT3 Mix from 1Step RT qPCR Kits
• Ready -to-use kit for cDNA sythesis prior to qPCR: #RTK01 - qScriber™ cDNA Synthesis Kit

--------------------------------------------------------------------

Tools for simplified Nucleic Acid extraction

• Proteinase K MGB Solution, 20 mg/ml for cell lysis and NA extraction needs
• Synthetic Carrier RNA (1 or 10 mg/ml Solution for precipitation of diluted NA samples

Contact us at info@highQu.com for Quotes and more information