highQu ready to use DNA ladders are mixtures of highly purified plasmid digests and PCR products. The high ladder purity allows for exceptional stability and room temperature storage. Ladders are ready to be directly loaded on agarose gels and are supplied with the loading dye solution for probe DNA. They provide sharp bands for DNA sizing and allow for approximate DNA quantification by comparing the amount of DNA of the band similar in size to your sample band. The most important factors influencing agarose gel electrophoresis results:  Use the right percentage of the agarose gel for the given size of the DNA to be analysed. Prepare the gel according to the instructions of the agarose manufacturer. Use the most sensitive nucleic acid stain (StainIN™ Green) in both agarose gel and in the buffer. Use the same loading dye both for the samples and for the DNA ladder. Select DNA ladder which is supplied with the loading dye solution for the DNA samples (Take5™ 1 kb DNA Ladder). For more precise sizing of the DNA, load the DNA ladder into the two slots of the agarose gel Use enough of the electrophoresis buffer to keep the agarose gel well-covered during the electrophoresis run.  Visualize the agarose gel to compare your sample bands with the ones of the DNA ladder using an optimal gel documentation system.

Our improved 2X 1Step RT qPCR Probe Kits provide highly sensitive target RNA detection when working with a wide variety of samples. The optimized 1Step RT qPCR Pro Mix in combination with a blend of thermostable Reverse Transcriptase and an advanced RNase Inhibitor (RT-Pro Mix) allows for a single step, one tube RT qPCR with great results even in multiplex reactions. The novel RT-Pro Mix ensures safe and efficient RNA template conversion into a single-stranded cDNA. Pure RNA samples, as well as lysed crude samples can serve as templates for one-step RT qPCR. Though the kit was not specifically designed for crude sample qPCR, it has a potential to work well for this application. The robust 1Step RT qPCR Pro Mix is a 2X concentrated qPCR master mix which includes dNTPs, buffer, and the hot start Taq DNA Polymerase. The Hot Start function ensures the highest sensitivity and specificity under both standard and fast qPCR cycling conditions. The kits provide excellent results on both AT and GC-rich templates and show early Ct values with minimum or no optimization. Our kits include PCR Water to guaranty the best performance. To suit the broad instrument range the 2X 1Step RT qPCR Probe Kits are available in three versions – without ROX, with low or high ROX concentration.

highQu PCRbeam™ Fast PCR Detection Kit is a convenient tool for fast detection of gene-specific amplification products obtained by PCR, LAMP or RPA. The detection is based on immunological reaction driven by Biotin and FITC (fluorescein isothiocyanate), thus the amplified DNA shall include Biotin and FITC labels. The PCR amplification has to be performed with one primer labeled with FITC at 5’-end and one primer labeled with Biotin at 5’-end. Alternatively the use of one of the labeled primers can be replaced by gene-specific FITC or Biotin labeled probe. Kit includes PCRbeam™ Membrane Strips that are coated with biotin-ligand on the test band and an anti-rabbit antibody on the control band. The bottom part of the strip which is used for sample application contains an anti-FITC antibody attached to gold particles. PCRbeam™ Detection Buffer is Tris-buffered saline enabling the detection. The PCRbeam™ Fast PCR Detection Kit can be applied for established tests or home-brew assays as a fast and sensitive yes/no detection method. The detection sensitivity is up to 100 fold higher than the one achievable with ethidium bromide stained gels what provides an environment friendly save and economical alternative to the use of mutagen stains. For establishing sensitive PCR-based tests before PCRbeam™ detection we recommend the use of hot-start PCR enzymes or master mixes, like highQu ALLin™ Hot Start Taq Mastermix or ALLin™ Hot Start Taq DNA Polymerase. Principle: The membrane strip is soaked for 10 minutes into the vial with the detection buffer mixed with PCR product. The lateral sample flow driven by gold particles moves the solution up the strip. FITC labeled DNA strand binds with the anti-FITC antibody on the gold particle and Biotin labeled DNA strand is caught by Biotin ligand attached to the test band. As both DNA strands remain hybridized at room temperature, the test band builds an aggregate that develops red-blue color. Excess gold particles that were not caught by FITC move up the strip and the anti-FITC antibody binds to the anti-rabbit antibody to develop the red-blue colored control band. If there is no PCR product in the reaction, then only the control band will be visible. If there is a specific product, the test band will be colored as well.

SecurRIN™ Advanced RNase Inhibitor is a premium tool for RNA protection from degradation during enzymatic reactions, storage or extraction. It is an extraordinary stable and robust enzyme: it works at up to 60°C temperature, remains active after weeks of room temperature exposure and multiple freezing thawing cycles. It is active in different buffer conditions within a broad pH range of 5.5 to 9.0, and within 0.5 - 1 mM concentration of DTT. SecurRIN™ Advanced RNase Inhibitor is a 50 kDa non-covalent inhibitor of RNase A, RNase B, and RNase C binding them in a 1 to 1 ratio. It is a recombinant protein derived from E. coli strain carrying human placenta RNase Inhibitor gene. SecurRIN™ Advanced RNase Inhibitor does not inhibit RNAses H, 1, T1, T2 and S1 Nuclease. It influences neither the activity nor the performance of DNA polymerases and of Reverse Transcriptases. The enzyme is free from DNAses and RNAses. Ribonuclease Inhibitor is a protein that protects RNA samples from degradation by ribonucleases. Ribonucleases are present everywhere in laboratory environment, they are very robust and ubiquitous enzymes that rapidly degrade RNA by destroying valuable RNA samples during molecular biology or diagnostics workflows. RNase inhibitor binds to RNases and inactivates these enzymes, ensuring the integrity and stability of RNA samples during such processes as RNA isolation, clean-up, cDNA synthesis, RT-qPCR and other applications. A good RNase inhibitor should have high affinity for RNases and strong inhibitory activity under a wide variety of buffer conditions. It shall tolerate different pH values, salt concentrations and shall have high affinity to different types of RNases. It should also be stable under higher temperatures and easy to use in all common RNA-related buffers with minimal interference with downstream applications. Furthermore, it is especially important, that the Inhibitor itself is pure and free from contaminating RNases. highQu offers SecurRIN™ Advanced RNase Inhibitor for RNA protection from degradation during enzymatic reactions, storage or extraction. Is is a 50 kDa non-covalent inhibitor of RNase A, RNase B, and RNase C.