OEM/COVID-19

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highQu RT qPCR Kits, available in 5 / 50 / 500 ml scale

  • Robust – inhibitor-resistant, work under a broad range of reaction conditions
  • Fast - designed for fast-cycling protocols, which enables fast test results
  • Outperforming – great results on complex templates, and in multiplexing

To enable adding much more sample into the RT qPCR reactions, we offer NEW 4X 1Step RT qPCR Probe Kits. These include a robust 4X qPCR master mix for high sample volume input of up to 10 µl in 20 µl reactions. The new kits are available with different ROX concentrations and detect virus RNA in very diluted samples as of <5 RNA copies per reaction. 4X 1Step RT qPCR Probe Kits have been tested for Sars-CoV-2 detection. Check Application Note for Sars-CoV-2 detection.

Our classical 1Step RT qPCR kits for a single step one tube RT qPCR from RNA templates include qPCR master mix and a blend of thermostable HighScriber™ Reverse Transcriptase with RNase Inhibitor. Kits can be supplied with water. For OEM customers, the kits can be supplied as they are or as separate components. Each component of the kit is available in 5 ml, 50 ml, 500 ml scale. 1Step RT qPCR Probe Kit versions with low or high ROX concentration are also available.

Product listing for one-step RT qPCR:

  • #QOP04 – 4X 1Step RT qPCR Probe Kit
  • #QOP05 – 4X 1Step RT qPCR Probe ROX L Kit
  • #QOP06 – 4X 1Step RT qPCR Probe ROX H Kit
  • #QOP04c1 – 1Step RT qPCR Probe Mix, 4X – special qPCR mix, designed for one-step kits, a component of #QOP04.
  • #QOP05c1 – 1Step RT qPCR Probe ROX L Mix, 4X – special qPCR mix for one-step kits, a component of #QOP05.
  • #QOP06c1 – 1Step RT qPCR Probe ROX H Mix, 4X – special qPCR mix for one-step kits, a component of #QOP06.
  • #QOP04c2 – RT4 Mix, 20X – RT enzyme blend, a component of the above-mentioned kits QOP04/5/6.
  • #QOP01 – 1Step RT qPCR Probe Kit
  • #QOP02 – 1Step RT qPCR Probe ROX L Kit
  • #QOP03 – 1Step RT qPCR Probe ROX H Kit
  • #QOP01c1 – 1Step RT qPCR Probe Mix, 2X – special qPCR mix, designed for one-step kits, a component of #QOP01.
  • #QOP02c1 – 1Step RT qPCR Probe ROX L Mix, 2X – special qPCR mix for one-step kits, a component of #QOP02.
  • #QOP03c1 – 1Step RT qPCR Probe ROX H Mix, 2X – special qPCR mix for one-step kits, a component of #QOP03.
  • #QOP01c2 – RT3 Mix, 20X – RT enzyme blend, a component of the above-mentioned kits QOP01/2/3.
  • #WAT01 – PCR Water, a component of all the above-mentioned kits.

highQu qPCR master mixes, available in 5 / 50 / 500 ml scale

  • Robust – inhibitor-resistant, work under a broad range of reaction conditions
  • Fast - designed for fast-cycling protocols, which enables fast test results
  • Outperforming – great results on complex templates, and in multiplexing

qPCR master mixes (can be combined in your own 1Step RT qPCR Kits) allow you to achieve the highest sensitivity under both standard and fast cycling conditions and provide excellent results on complex templates with minimum or no optimization.

Listing of qPCR Master mixes:

highQu dNTPs available in 1 ml, 25 ml, 100 ml, and larger scale

  • ≥99% HPLC-confirmed purity
  • Pure from contaminating & deaminated nucleotides, pyrophosphates
  • Free from nucleases & proteases, from bacterial & human DNA
  • Functionally tested in demanding applications
  • Stable during storage/usage & multiple freezing/thawing

highQu dNTPs Product Listing:

  • #NUM25B a 25 mM dNTP Mix of all 4 dNTPs in 1 ml, 25 ml, 100 ml and liter scale (PI example).
  • #NUCA dATP, 100 mM Sodium Salt Solution, in 1 ml, 25 ml, 100 ml and liter scale.
  • #NUCC dCTP, 100 mM Sodium Salt Solution, in 1 ml, 25 ml, 100 ml and liter scale.
  • #NUCG dGTP, 100 mM Sodium Salt Solution, in 1 ml, 25 ml, 100 ml and liter scale.
  • #NUCT dTTP, 100 mM Sodium Salt Solution, in 1 ml, 25 ml, 100 ml and liter scale.
  • #NUCU dUTP, 100 mM Sodium Salt Solution, in 1 ml, 25 ml, 100 ml and liter scale.

Other basic reagents for diagnostic kit production and development

 

Inquire: +49 7250 33 13 401 - info@highQu.com