The ALLin™ Hot Start Bst Polymerase is a recombinant protein, representing a large fragment of the B. stearothermophilus DNA Polymerase expressed in E. coli cells. The activity of the enzyme is blocked at ambient temperature due to molecular inhibition based hot-start technology, and the polymerase is activated only at 45-50°C what reduces non-specific amplification, primer dimer formation and is therefore excellent for ambient reaction setup. This robust polymerase with a strong strand displacement activity and high temperature tolerance ensures high amplification yield at constant temperature when working with impure or low-copy number targets as well as with complex templates. The Allin™ Bst Buffer includes optimal concentrations of magnesium and dNTPs, what minimizes pipetting steps. This ALLin™ Hot Start Bst Polymerase-buffer system together with a supplied enhancer, detects <3 DNA targets in a short time without the use of a thermocycler. This glycerol-free Hot Start Bst Polymerase is also an excellent tool for development of dry format lyophilized kits for pathogen detection using isothermal amplification techniques.

For more convenience, we recommend our kit formulations:

ALLin™ HS Isothermal DNA Amplification Kit and ALLin™ HS Iso-Colorimetric DNA Amplification Kit both including 2X master mix formulations.

Technical characteristics of Hot Start Bst Polymerase (large fragment):

·  The polymerase is blocked at ambient temperature and activated at temperatures above 45°C.

·  Strong 5’ - 3’ strand displacement activity

·  Strong 5’ - 3’ polymerase activity

·  No 5’ - 3’ exonuclease activity

·  No 3’ - 5’ exonuclease (proofreading) activity

·  Only minor reverse transcriptase activity

·  Optimal amplification temperature is 65°C

·  Working temperature range is 55-70°C

·  Optimal reaction time is 20 minutes (depends on the buffer)

·  If needed, the reaction can be extended to 30 - 60 minutes

·  The enzyme is inactivated in 10 minutes at 80°C

The use of this product in certain applications may be covered by patents of third parties. The user has to analyse all applicable Limited Use Label Licenses and may need licensing from third parties.