Accelerate PCR genotyping: amplify crude sample without DNA extraction using SampleIN Direct PCR Kit
SampleIN™ Direct PCR Kit is the great tool to perform fast PCR without template DNA extraction
|Fast cycling||GC/AT rich PCR||Max product||High Yields||Direct PCR||Multiplex PCR||Direct gel loading|
|Fast, direct crude sample PCR, includes ALLin™ HS Taq DNA Polymerase in 2X mastermix with red loading dye (available separately)||●||●||●||5 kb||●||●●●||●||●|
|Robust Taq with ALLin™ buffer for standard, fast, GC rich PCR||●||●||●||6 kb||●●||●||●|
|ALLin™ Taq DNA Polymerase in 2X mastermix with loading dye||●||●||●||6 kb||●●||●||●||●|
|ALLin™ Taq DNA Polymerase in 2X mastermix||●||●||●||6 kb||●●||●||●|
|Classical Taq, magnesium supplied separately||5 kb||●|
Tips for the best direct PCR or crude sample PCR amplification results:
• If direct PCR amplification does not work, reduce the sample input amount, for example use less of bacterial colony material or less of tissue.
• For some samples you might need performing a simple lysis with proteinase K, lysozyme or commercially available direct PCR amplification lysis buffers.
• Try different dilutions of the lysed sample for the PCR amplification.
• Choose primers with high specificity and efficiency for the target sequence to ensure successful amplification.
• Use only the hot-start DNA polymerase or master mix for the best amplification result when working with crude samples.
• Determine the optimal annealing temperature by conducting a temperature gradient PCR to enhance primer binding and specificity.
• Extend the denaturation step to ensure complete DNA denaturation, especially in crude samples with potentially more inhibitors that can be degraded when heating.
• Include positive control (known DNA template/primers) and negative controls (no template) to validate the PCR amplification results.