The HighScriber™ Reverse Transcriptase Mix is a premium tool for the high efficiency reverse transcription of up to 12-15 kb long cDNA. Mix includes HighScriber™ Reverse Transcriptase and Ribonuclease Inhibitor for save, robust cDNA synthesis and ease of use. HighScriber™ Reverse Transcriptase allows for high detection sensitivity from 1 pg of total RNA. The wide reaction temperature range (38°C - 55°C) ensures efficient cDNA synthesis from complex or GC rich templates. The enzyme uses ssRNA or ssDNA as a template, possesses no detectable Ribonuclease H activity specific to RNA in RNA-DNA hybrids. A highly reduced Ribonuclease H activity allows for transcription of full lengths long transcripts. HighScriber™ Reverse Transcriptase can be used for RACE as it has terminal transferase activity - adds cytosines to 3’ ends of cDNA. The Ribonuclease inhibitor premixed with the RT ensures RNA protection from ribonuclease degradation. Supplied 5X ALLin™ HighScriber Buffer includes everything you need for the cDNA synthesis reaction. To minimize pipetting steps it contains MgCl2, dNTPs, enhancers, stabilizers. The only things to add is the template RNA and primer.

Our improved 2X 1Step RT qPCR Probe Kits provide highly sensitive target RNA detection when working with a wide variety of samples. The optimized 1Step RT qPCR Pro Mix in combination with a blend of thermostable Reverse Transcriptase and an advanced RNase Inhibitor (RT-Pro Mix) allows for a single step, one tube RT qPCR with great results even in multiplex reactions. The novel RT-Pro Mix ensures safe and efficient RNA template conversion into a single-stranded cDNA. Pure RNA samples, as well as lysed crude samples can serve as templates for one-step RT qPCR. Though the kit was not specifically designed for crude sample qPCR, it has a potential to work well for this application. The robust 1Step RT qPCR Pro Mix is a 2X concentrated qPCR master mix which includes dNTPs, buffer, and the hot start Taq DNA Polymerase. The Hot Start function ensures the highest sensitivity and specificity under both standard and fast qPCR cycling conditions. The kits provide excellent results on both AT and GC-rich templates and show early Ct values with minimum or no optimization. Our kits include PCR Water to guaranty the best performance. To suit the broad instrument range the 2X 1Step RT qPCR Probe Kits are available in three versions – without ROX, with low or high ROX concentration.

SecurRIN™ Advanced RNase Inhibitor is a premium tool for RNA protection from degradation during enzymatic reactions, storage or extraction. It is an extraordinary stable and robust enzyme: it works at up to 60°C temperature, remains active after weeks of room temperature exposure and multiple freezing thawing cycles. It is active in different buffer conditions within a broad pH range of 5.5 to 9.0, and within 0.5 - 1 mM concentration of DTT. SecurRIN™ Advanced RNase Inhibitor is a 50 kDa non-covalent inhibitor of RNase A, RNase B, and RNase C binding them in a 1 to 1 ratio. It is a recombinant protein derived from E. coli strain carrying human placenta RNase Inhibitor gene. SecurRIN™ Advanced RNase Inhibitor does not inhibit RNAses H, 1, T1, T2 and S1 Nuclease. It influences neither the activity nor the performance of DNA polymerases and of Reverse Transcriptases. The enzyme is free from DNAses and RNAses. Ribonuclease Inhibitor is a protein that protects RNA samples from degradation by ribonucleases. Ribonucleases are present everywhere in laboratory environment, they are very robust and ubiquitous enzymes that rapidly degrade RNA by destroying valuable RNA samples during molecular biology or diagnostics workflows. RNase inhibitor binds to RNases and inactivates these enzymes, ensuring the integrity and stability of RNA samples during such processes as RNA isolation, clean-up, cDNA synthesis, RT-qPCR and other applications. A good RNase inhibitor should have high affinity for RNases and strong inhibitory activity under a wide variety of buffer conditions. It shall tolerate different pH values, salt concentrations and shall have high affinity to different types of RNases. It should also be stable under higher temperatures and easy to use in all common RNA-related buffers with minimal interference with downstream applications. Furthermore, it is especially important, that the Inhibitor itself is pure and free from contaminating RNases. highQu offers SecurRIN™ Advanced RNase Inhibitor for RNA protection from degradation during enzymatic reactions, storage or extraction. Is is a 50 kDa non-covalent inhibitor of RNase A, RNase B, and RNase C.  

highQu qPCR master mixes are based on the small molecular inhibitor technology Hot Start PCR allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions. They provide excellent results on both AT and GC rich templates, in multiplexing and guaranty rapid extension with early Ct values with minimum or no optimization. Our master mixes are supplied with PCR Water to guaranty the best performance. To suit the broad instrument range the ORA™ qPCR Probe Master mixes are available in three versions – without ROX, with low or high ROX concentration.