highQu ready to use DNA ladders are mixtures of highly purified plasmid digests and PCR products. The high ladder purity allows for exceptional stability and room temperature storage. Ladders are ready to be directly loaded on agarose gels and are supplied with the loading dye solution for probe DNA. They provide sharp bands for DNA sizing and allow for approximate DNA quantification by comparing the amount of DNA of the band similar in size to your sample band. The most important factors influencing agarose gel electrophoresis results:  Use the right percentage of the agarose gel for the given size of the DNA to be analysed. Prepare the gel according to the instructions of the agarose manufacturer. Use the most sensitive nucleic acid stain (StainIN™ Green) in both agarose gel and in the buffer. Use the same loading dye both for the samples and for the DNA ladder. Select DNA ladder which is supplied with the loading dye solution for the DNA samples (Take5™ 1 kb DNA Ladder). For more precise sizing of the DNA, load the DNA ladder into the two slots of the agarose gel Use enough of the electrophoresis buffer to keep the agarose gel well-covered during the electrophoresis run.  Visualize the agarose gel to compare your sample bands with the ones of the DNA ladder using an optimal gel documentation system.

highQu ALLin™ Taq DNA Polymerase is the versatile engineered enzyme which in combination with the optimized ALLin™ buffer provides higher success rates in demanding PCR applications like amplification of complex templates, crude sample PCR and fast cycling. ALLin™ Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 10^4 (a number of correct nucleotides incorporated before the error occurs) and produces A-tailed products suitable for ligating into TA cloning vectors. The convenience of ALLin™ Taq DNA Polymerase is maximized by the use of 2X Red Mastermix providing the additional advantage of reduced pipetting and minimized errors. ALLin™ Red Taq Mastermix, 2X is premixed with red dye and density reagents for direct loading on the gels after the PCR. In a 2% agarose TAE gel the dye migrates with~350 bp DNA, in 1% agarose TAE gel with ~ 600 bp DNA fragments. The mastermix is even supplied with the PCR Water, and the only thing to add is the template with primers.

Proteinase K (Molecular Biology Grade Solution) is a serine peptidase with a very high specific activity and a broad spectrum of protein digestion possibilities. The solution is designed to be used for protein degradation (up to tetrapeptides) during the cell lysis and RNA/DNA extraction procedures under hush reaction conditions such as the higher temperatures and the presence of detergents. The enzyme efficiently degrades DNases and RNases during nucleic acid isolation process. The high purity of the Proteinase K and controlled absence of both DNAse and RNase contamination ensures the integrity of nucleic acids. The 100% enzyme activity (when stored at -20°C) is guaranteed for at least two years after production. However, the experiments proved that the proteinase remains close to 90% active even when stored at +37°C for 18 months. The enzyme is supplied as a 20 mg/ml concentrated solution with an average specific activity of more than 800 u/ml. Active in all common buffers used for cell lysis and RNA/DNA extraction, in a presence of urea, SDS and guanidinium salts Stable at high temperature of up to 56°C Can be inactivated by heating at 65oC for 20 minutes or at 75oC for 10 minutes Active in a pH range of 4–12 with an optimum pH 7.5–8.0 The Proteinase K gene from album expressed in yeast host. The quality limit of allowed host DNA presence is ≤ 0.25 pg/U measured by qPCR what is about a half when compared to other suppliers. Unit Definition Folin & Ciocalteu’s method - One unit is required to hydrolyze urea-denaturated hemoglobin producing color equivalent of 1 μmol tyrosine in 1 minute at 37°C and pH 7.5, 1 U = 1 m Anson U.

highQu Synthetic Carrier RNA is designed to be used in all kind nucleic acid purification and precipitation procedures as a carrier and co-precipitant of nucleic acids. It is especially useful to increase the amount of RNA or DNA pellet in low concentrated solutions, in such procedures, as viral RNA extraction from human specimen samples. In contrast to commonly used carrier RNAs such as tRNA, yeast RNA, or sonicated salmon sperm DNA, the Synthetic Carrier RNA is free from animal or yeast RNA contamination. Coprecipitated RNA and DNA can be directly used for all common downstream applications, such as PCR or RT-PCR, as well as highly sensitive qPCR. The use of carrier RNAs for coprecipitation of nucleic acids may interfere with spectrophotometrical concentration measurements. The presence of carrier RNAs in the RNA or DNA solution may have some influence on certain enzymatic reactions performed by such enzymes that act on all nucleic acid molecules, for example T4 Polynucleotide Kinase or Terminal DNA Transferase.

highQu PCR Water is a supplementary high quality reagent for all demanding PCR and qPCR and other molecular biology applications. It saves time being on your bench and guaranties the purity of reactions and inhibition-free performance of PCR reagents. highQu PCR Water is a deionized, membrane filtered water continuously tested in ultrasensitive qPCR and PCR applications, in amplification of long targets and highly specific detection of few copies of templates.

Derived from our HiFi polymerase, the highQu ALLin™ Mega HiFi DNA Polymerase provides much lower error rate PCR with a 100 higher fidelity compared to Taq. The ALLin™ Mega HiFi DNA Polymerase is engineered to be much faster and to generate a higher yield of long PCR products up to 20 kb from complex GC-rich templates. Therefore the ALLin™ Mega HiFi DNA Polymerase is an excellent choice for longer and very complex PCR applications where the highest fidelity is demanded. It is an enzyme of choice for cloning and all kind of sequencing applications including NGS. Generated blunt-ended PCR products are suitable for ligation into blunt vectors. For maximum convenience, the ALLin™ Mega HiFi Mastermix, 2X (HLM0201) and ALLin™ Mega HiFi Red Mastermix, 2X (HLM0301) are available. Pfu DNA Polymerase was originally found in thermophilic bacterium Pyrococcus furiosus. It is a thermostable DNA polymerase that was widely used in molecular biology applications for its high fidelity of DNA amplification due to a high proofreading activity. Pfu polymerase has 3' to 5' exonuclease proofreading activity, which enables it to correct errors that occur during DNA synthesis. The enzyme is commonly used for cloning and sequencing applications, however it is relatively slow, sensitive to contaminants and inhibitors and has low processivity resulting in usually low PCR yields. Alternative, mostly better DNA polymerases have been developed by different companies to address the limitations of Pfu polymerase. These include such enzymes as Phusion® DNA polymerase, Q5® DNA polymerase from NEB, and many others. These enzymes offer improved elongation rates, reduced error rates, and increased efficiency for NGS applications. The ALLin™ MEGA HiFi Polymerase offered by highQu provides 100X higher fidelity compared to Taq Polymerase, which is much higher than Pfu. In addition, the hot start enzyme version allows to reduce the background to the minimum. Such robust enzyme offers not only precise amplification, but also enables PCR from very complex templates and generation of very long PCR products up to 30 kb and above. Availability of faster, more robust and more precise polymerases influenced successful development of next generation sequencing techniques (NGS) where PCR precision and yield is crucial for reliable sequencing results.