highQu ready to use DNA ladders are mixtures of highly purified plasmid digests and PCR products. The high ladder purity allows for exceptional stability and room temperature storage. Ladders are ready to be directly loaded on agarose gels and are supplied with the loading dye solution for probe DNA. They provide sharp bands for DNA sizing and allow for approximate DNA quantification by comparing the amount of DNA of the band similar in size to your sample band. The most important factors influencing agarose gel electrophoresis results:  Use the right percentage of the agarose gel for the given size of the DNA to be analysed. Prepare the gel according to the instructions of the agarose manufacturer. Use the most sensitive nucleic acid stain (StainIN™ Green) in both agarose gel and in the buffer. Use the same loading dye both for the samples and for the DNA ladder. Select DNA ladder which is supplied with the loading dye solution for the DNA samples (Take5™ 1 kb DNA Ladder). For more precise sizing of the DNA, load the DNA ladder into the two slots of the agarose gel Use enough of the electrophoresis buffer to keep the agarose gel well-covered during the electrophoresis run.  Visualize the agarose gel to compare your sample bands with the ones of the DNA ladder using an optimal gel documentation system.

highQu ALLin™ Taq DNA Polymerase is the versatile engineered enzyme which in combination with the optimized ALLin™ buffer provides higher success rates in demanding PCR applications like amplification of complex templates, crude sample PCR and fast cycling. ALLin™ Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 10^4 (a number of correct nucleotides incorporated before the error occurs) and produces A-tailed products suitable for ligating into TA cloning vectors. The convenience of ALLin™ Taq DNA Polymerase is maximized by the use of 2X Red Mastermix providing the additional advantage of reduced pipetting and minimized errors. ALLin™ Red Taq Mastermix, 2X is premixed with red dye and density reagents for direct loading on the gels after the PCR. In a 2% agarose TAE gel the dye migrates with~350 bp DNA, in 1% agarose TAE gel with ~ 600 bp DNA fragments. The mastermix is even supplied with the PCR Water, and the only thing to add is the template with primers.

highQu ORA™ qPCR Probe Mixes and 1Step RT qPCR Probe Kits are available in 3 versions – without ROX, with low or high ROX concentration. ROX is a passive reference dye for qPCR, which is used to normalize data in quantitative real-time PCR (qPCR) experiments. The ROX for qPCR Mixes, 50 μM is a great choice for those users who wish to have the universal Probe mix, that can be used either without ROX, or with different ROX concentrations, what allows the applications on all kind of available real time PCR instruments.ROX for qPCR Mixes, 50 μM can be added directly to the qPCR master mix no matter if it is 2X or 4X concentrated.Mixture can be further stored as recommended by the master mix provider, and used as requested by the qPCR mix protocol and instrument instructions.For optional use, the ROX passive reference dye can be premixed within the qPCR Mixes. If the purchaser has an instrument capable of optional ROX detection and wishes to perform the optional normalization of the signal, then the user must select the option in the software. Notice to Purchaser: With purchasing of this product, no rights are conveyed with respect to U.S. Patent: 5,928,907 and corresponding patents outside the US.highQu qPCR mixes and kits that might be premixed with ROX dye if requiredCodeProduct NameDQP01SampleIN™ Direct qPCR Probe Mix, 4XDOS01SampleIN™ 1Step RTqPCR Probe Mix, 4XQPP01ORA™ qPCR Probe Mix, 2XQPP04ORA™ SEE qPCR Probe Mix, 2XQOP112X 1Step RT qPCR Probe Kit (with 2X qPCR Mix)QOP044X 1Step RT qPCR Probe Kit (with 4X qPCR Mix)

Proteinase K (Molecular Biology Grade Solution) is a serine peptidase with a very high specific activity and a broad spectrum of protein digestion possibilities. The solution is designed to be used for protein degradation (up to tetrapeptides) during the cell lysis and RNA/DNA extraction procedures under hush reaction conditions such as the higher temperatures and the presence of detergents. The enzyme efficiently degrades DNases and RNases during nucleic acid isolation process. The high purity of the Proteinase K and controlled absence of both DNAse and RNase contamination ensures the integrity of nucleic acids. The 100% enzyme activity (when stored at -20°C) is guaranteed for at least two years after production. However, the experiments proved that the proteinase remains close to 90% active even when stored at +37°C for 18 months. The enzyme is supplied as a 20 mg/ml concentrated solution with an average specific activity of more than 800 u/ml. Active in all common buffers used for cell lysis and RNA/DNA extraction, in a presence of urea, SDS and guanidinium salts Stable at high temperature of up to 56°C Can be inactivated by heating at 65oC for 20 minutes or at 75oC for 10 minutes Active in a pH range of 4–12 with an optimum pH 7.5–8.0 The Proteinase K gene from album expressed in yeast host. The quality limit of allowed host DNA presence is ≤ 0.25 pg/U measured by qPCR what is about a half when compared to other suppliers. Unit Definition Folin & Ciocalteu’s method - One unit is required to hydrolyze urea-denaturated hemoglobin producing color equivalent of 1 μmol tyrosine in 1 minute at 37°C and pH 7.5, 1 U = 1 m Anson U.

highQu Synthetic Carrier RNA is designed to be used in all kind nucleic acid purification and precipitation procedures as a carrier and co-precipitant of nucleic acids. It is especially useful to increase the amount of RNA or DNA pellet in low concentrated solutions, in such procedures, as viral RNA extraction from human specimen samples. This product is entirely synthetic in origin. It consists of an aqueous solution of polyinosinic acid at concentrations of 1 mg/mL or 10 mg/mL. The polymer is heterogeneous in length, likely comprising chains ranging from several hundred up to approximately one thousand nucleotides. In contrast to commonly used carrier RNAs such as tRNA, yeast RNA, or sonicated salmon sperm DNA, the Synthetic Carrier RNA is free from animal or yeast RNA contamination. Coprecipitated RNA and DNA can be directly used for all common downstream applications, such as PCR or RT-PCR, as well as highly sensitive qPCR . The use of carrier RNAs for coprecipitation of nucleic acids may interfere with spectrophotometrical concentration measurements. The presence of carrier RNAs in the RNA or DNA solution may have some influence on certain enzymatic reactions performed by such enzymes that act on all nucleic acid molecules, for example T4 Polynucleotide Kinase or Terminal DNA Transferase.   highQu Synthetic Carrier RNA: A Guide to Low-Input RNA and DNA Recovery Introduction Recovering very small amounts of RNA or DNA can be difficult in standard extraction and precipitation workflows. When nucleic acid concentrations are low, valuable material may remain in the supernatant, stick to plastic surfaces, or form pellets too small to recover efficiently. highQu Synthetic Carrier RNA is designed to solve this problem. This aqueous polyinosinic acid solution acts as a carrier during nucleic acid purification, helping improve recovery from dilute, degraded, or trace-input samples. It is especially useful in viral RNA extraction, low-copy nucleic acid workflows, and other protocols where maximizing yield is critical. This article explains what highQu Synthetic Carrier RNA does, why it improves recovery, when to use it, and best practices for integrating it into RNA and DNA extraction workflows What Is highQu Synthetic Carrier RNA? highQu Synthetic Carrier RNA is a synthetic carrier reagent used to improve the recovery of low-input nucleic acids during extraction, cleanup, and precipitation procedures. Its primary role is to support the recovery of trace amounts of RNA or DNA when the target material is too limited to precipitate or bind efficiently on its own. Unlike target nucleic acids used for downstream analysis, carrier RNA is not intended to function as a diagnostic analyte or biological sample component. Instead, it acts as a recovery aid that improves extraction efficiency and minimizes sample loss Main Uses of highQu Synthetic Carrier RNA Viral RNA Extraction One of the most common applications for carrier RNA is viral RNA extraction. Viral samples often contain extremely low amounts of nucleic acid, making efficient recovery essential for downstream detection methods such as RT-qPCR. Adding carrier RNA can improve: RNA recovery efficiency Pellet visibility Binding performance in column-based systems Sample retention during wash and transfer steps This makes highQu Synthetic Carrier RNA particularly valuable in diagnostic and research workflows involving low viral loadsL Low-Input RNA Cleanup RNA cleanup workflows frequently involve dilute or partially degraded samples. In these cases, nucleic acid loss during purification can significantly reduce final yield. Carrier RNA helps stabilize recovery by increasing the total nucleic acid mass during precipitation or binding. This improves the likelihood that small RNA quantities remain recoverable throughout the workflow. Common applications include: RNA extracted from limited biological material Environmental RNA samples Degraded RNA preparations Trace RNA purification DNA Recovery and Precipitation Although commonly associated with RNA workflows, carrier RNA can also improve low-input DNA recovery. In DNA precipitation protocols, especially with small or fragmented samples, carrier RNA supports co-precipitation and helps form visible pellets that are easier to handle. This can improve recovery from: Low-copy DNA samples Fragmented DNA preparations Dilute DNA solutions Cleanup steps following enzymatic reactions Why Carrier RNA Improves Nucleic Acid Recovery Improved Pellet Formation When nucleic acid concentrations are very low, precipitation can become inefficient. Pellets may be too small to see or unstable during centrifugation and washing. Carrier RNA provides additional mass that helps nucleic acids aggregate more effectively during precipitation. As a result, workflows benefit from: Better pellet formation Reduced sample loss Improved transfer efficiency Greater recovery consistency Reduced Loss During Purification Small amounts of RNA or DNA can easily adhere to tube walls, pipette tips, or remain suspended in the supernatant. Carrier RNA helps minimize these losses by supporting stable nucleic acid recovery throughout: Centrifugation Alcohol precipitation Wash steps Transfer procedures Its value is practical rather than analytical: it helps ensure the nucleic acid remains recoverable inside the tube. Best Workflow Tips for Using Carrier RNA Add Carrier RNA Early in the Workflow For best results, add HighQu Synthetic Carrier RNA: Before alcohol precipitation steps, or Early during lysis or binding in column-based extraction protocols Early addition allows the carrier to participate fully in nucleic acid aggregation and recovery. Use It for Low-Input or Degraded Samples Carrier RNA is most beneficial when working with: Trace RNA Low-copy DNA Dilute samples Degraded nucleic acids Diagnostic extraction workflows High-input samples generally do not require carrier RNA because the target nucleic acid already precipitates efficiently. Consider Quantification Methods Carefully Carrier RNA can affect absorbance-based quantification methods such as spectrophotometry. Because the carrier contributes additional nucleic acid signal, total concentration readings may appear artificially elevated. For more accurate downstream measurement, fluorescence-based quantification methods are often preferred when carrier RNA is present. When Should You Use HighQu Synthetic Carrier RNA? HighQu Synthetic Carrier RNA is especially useful for workflows involving: Viral RNA extraction Low-input RNA purification Low-input DNA precipitation Recovery from dilute samples Recovery from fragmented or degraded nucleic acids Protocols where pellet visibility matters It is less necessary for high-yield samples where nucleic acids already recover efficiently without assistance. Applications Where Carrier RNA Is Not Intended highQu Synthetic Carrier RNA is a RUEO - research use only product, it is not designed for: Protein purification workflows Antibody production Vaccine adjuvant applications Acting as a biological analyte or detection target Its purpose is strictly to improve nucleic acid recovery efficiency in extraction and precipitation workflows. Recommendations for Best Results To maximize reproducibility and recovery efficiency: Use consistent carrier RNA amounts across samples Add the carrier at the same workflow stage each time Pair with fluorescence-based quantification when accurate concentration measurement is critical Validate downstream compatibility with sensitive assays when optimizing protocols When sample scarcity is the main challenge, carrier RNA can substantially improve the likelihood of recovering usable RNA or DNA. Frequently Asked Questions (FAQ) What is HighQu Synthetic Carrier RNA used for? HighQu Synthetic Carrier RNA is used to improve recovery of low-input RNA and DNA during extraction, cleanup, and precipitation workflows. How does carrier RNA improve RNA extraction? Carrier RNA increases the total nucleic acid mass during purification, which helps improve pellet formation and reduces sample loss during centrifugation, washing, and transfers. When should carrier RNA be added? Carrier RNA is typically added before alcohol precipitation or early in the lysis/binding step of column-based extraction protocols. Can carrier RNA be used for DNA recovery? Yes. Although commonly used in RNA workflows, carrier RNA can also improve recovery of low-input or fragmented DNA during precipitation and cleanup steps. Does carrier RNA affect qPCR or nucleic acid quantification? Carrier RNA can influence absorbance-based measurements and may increase apparent nucleic acid concentration readings. Fluorescence-based quantification methods are often more accurate when carrier RNA is present. Is carrier RNA necessary for high-input samples? Usually not. High-input samples generally recover efficiently without carrier assistance. Carrier RNA is most valuable when working with trace or dilute nucleic acid samples. Conclusion highQu Synthetic Carrier RNA is a practical solution for improving nucleic acid recovery in low-input workflows. By supporting pellet formation and minimizing loss during purification, it helps researchers recover RNA and DNA more consistently from dilute, fragmented, or difficult samples. Its strongest applications include viral RNA extraction, low-copy nucleic acid recovery, and workflows where every bit of yield matters. When used correctly and consistently, carrier RNA can significantly improve extraction reliability without altering the biological target itself.

highQu PCR Water is a supplementary high quality reagent for all demanding PCR and qPCR and other molecular biology applications. It saves time being on your bench and guaranties the purity of reactions and inhibition-free performance of PCR reagents. highQu PCR Water is a deionized, membrane filtered water continuously tested in ultrasensitive qPCR and PCR applications, in amplification of long targets and highly specific detection of few copies of templates.

Derived from our HiFi polymerase, the highQu ALLin™ Mega HiFi DNA Polymerase provides much lower error rate PCR with a 100 higher fidelity compared to Taq. The ALLin™ Mega HiFi DNA Polymerase is engineered to be much faster and to generate a higher yield of long PCR products up to 20 kb from complex GC-rich templates. Therefore the ALLin™ Mega HiFi DNA Polymerase is an excellent choice for longer and very complex PCR applications where the highest fidelity is demanded. It is an enzyme of choice for cloning and all kind of sequencing applications including NGS. Generated blunt-ended PCR products are suitable for ligation into blunt vectors. For maximum convenience, the ALLin™ Mega HiFi Mastermix, 2X (HLM0201) and ALLin™ Mega HiFi Red Mastermix, 2X (HLM0301) are available. Pfu DNA Polymerase was originally found in thermophilic bacterium Pyrococcus furiosus. It is a thermostable DNA polymerase that was widely used in molecular biology applications for its high fidelity of DNA amplification due to a high proofreading activity. Pfu polymerase has 3' to 5' exonuclease proofreading activity, which enables it to correct errors that occur during DNA synthesis. The enzyme is commonly used for cloning and sequencing applications, however it is relatively slow, sensitive to contaminants and inhibitors and has low processivity resulting in usually low PCR yields. Alternative, mostly better DNA polymerases have been developed by different companies to address the limitations of Pfu polymerase. These include such enzymes as Phusion® DNA polymerase, Q5® DNA polymerase from NEB, and many others. These enzymes offer improved elongation rates, reduced error rates, and increased efficiency for NGS applications. The ALLin™ MEGA HiFi Polymerase offered by highQu provides 100X higher fidelity compared to Taq Polymerase, which is much higher than Pfu. In addition, the hot start enzyme version allows to reduce the background to the minimum. Such robust enzyme offers not only precise amplification, but also enables PCR from very complex templates and generation of very long PCR products up to 30 kb and above. Availability of faster, more robust and more precise polymerases influenced successful development of next generation sequencing techniques (NGS) where PCR precision and yield is crucial for reliable sequencing results.