New Products

NEW since November 2021: Enzymes and kits for Isothermal Amplification needs.

NEW since June 2021: 4X 1Step Probe RT-qPCR Kits include 4X qPCR mix for the highest sample volume input.

New since November 2021: Isothermal Amplification Enzymes and Kits

Isothermal amplification of nucleic acids based on performance of such strand displacement polymerases as phi29 or Bst is a powerful and simple method to detect and amplify low abundance DNA and RNA targets within a short period of time and without the thermal cycler. highQu introduces a smart selection of enzymes and kits for isothermal amplification needs.

This short guide will help you to select the right product depending on your applications

  • ALLin™ Isothermal DNA Amplification Kit  is to be used mainly for Isothermal DNA amplification at 70°C, LAMP. Works on DNA templates and includes mix with buffer, dNTPs, Bst Polymerase, dye for optional real-time detection, and water.
  • ALLin™ Isothermal 1Step RNA  Amplification Kit is to be used for Virus RNA detection, SARS-CoV-2 detection, Isothermal  RNA amplification, RT-LAMP. Works on RNA templates and includes Reverse transcription mix, amplification mix with buffer, dNTPs, Bst Polymerase, the dye for optional real-time detection, and water.
  • phi29 DNA Polymerase is a classical recombinant enzyme supplied with 10X buffer and dedicated for use on DNA templates in such applications as isothermal DNA amplification at 30°C, RCA, WGA, MDA and many more.

ASK FOR A SAMPLE RIGHT NOW

NEW ALLin™ Isothermal DNA Amplification Kit 

ALLin™ Isothermal DNA Amplification Kit enables detection of as little as 5 target DNA molecules in a short time of 20 minutes even without the use of a thermal cycler. The Kit includes a 2X master mix with optimized high-performance buffer, dNTPs and a recombinant Bst Polymerase large fragment having strong 5’ - 3’ strand displacement activity and efficient 5’-3’ polymerase activity working at 55-70°C.

More information: Product Insert ALLin™ Isothermal DNA Amplification Kit

Applications

  • Isothermal DNA amplification at elevated temperature
  • Real-time detection of DNA amplification
  • LAMP - loop-mediated isothermal amplification
  • WGA - whole genome amplification

Benefits

  • Efficient 20 minutes DNA amplification at constant 55-70°C temperature
  • ALLin™ format, supplied with water and a dye for fast real-time detection
  • Bst DNA Polymerase with strong strand displacement activity
  • Robust on complex templates and crude samples
  • Low-copy (<5 molecules) targets detection

Isothermal DNA Amplification Kit

NEW ALLin™ Isothermal 1Step RNA  Amplification Kit 

ALLin™ Isothermal 1Step RNA Amplification Kit enables detection of < 5 target RNA molecules in a short time of 30 minutes without the use of PCR cycler.  An RT5 Mix, the blend of a thermostable reverse transcriptase and highly efficient RNase Inhibitor is included in the kit to initially synthesize cDNA from RNA templates. The cDNA is amplified by Bst polymerase. Kit includes a 2X master mix with optimized high-performance buffer, dNTPs and a recombinant Bst Polymerase large fragment having strong 5’ - 3’ strand displacement activity and efficient 5’-3’ polymerase activity working at 55-70°C.

More: Product Insert ALLin™ Isothermal 1Step RNA Amplification Kit

Applications

  • Virus RNA detection, SARS-CoV-2 detection
  • Isothermal one step RNA amplification at elevated temperature
  • RT-LAMP – reverse transcription loop-mediated isothermal amplification

Benefits

  • Efficient 30 minutes 1 Step RNA amplification at 55-70°C temperature
  • ALLin™ format, supplied with water and a dye for fast real-time detection
  • Bst DNA polymerase with strong strand displacement activity
  • Thermostable reverse transcriptase blended with RNase inhibitor
  • Robust on complex RNA templates and crude samples

Isothermal 1Step RNA Amplification Kit

NEW phi29 DNA Polymerase

Recombinant phi29 DNA Polymerase is a classical enzyme dedicated for use in common isothermal DNA amplification applications that are carried out at moderate temperature based on a strand displacement activity. The enzyme is supplied with an optimized high-performance buffer. The user has to add dNTPs, template and primers.

More: Product Insert phi29 DNA Polymerase

Applications

  • Isothermal DNA amplification for sequencing, cloning
  • RCA – rolling cycle amplification
  • MDA – multiple displacement amplification
  • WGA - whole genome amplification
  • Protein - primed or RNA-primed DNA amplification

Benefits

  • High yield DNA amplification at constant 30°C temperature
  • Robust polymerase with strong strand displacement activity
  • High processivity, synthesis of over 70 kb long DNA strains
  • High fidelity, low error rate for sequencing and cloning
  • Classical enzyme/buffer formulation for known phi29 applications

 

***************************

 

New since June 2021: 4X 1Step Probe RT-qPCR Kits that include 4X qPCR mix for highest sample volume input

Our classical 1Step RT qPCR Probe Kits are very popular products for Sars-CoV-2 detection needs. However, scientists who work with low copy number Sars-CoV-2 RNA samples requested to have 4X concentrated qPCR mixes in these kits instead of 2X qPCR mixes.
NEW 4X 1Step Probe RT-qPCR Kit versions have been developed for more sample input, enabling working with highly diluted low-copy-number samples. They include a 4X concentrated qPCR master mix, that allows adding up to 10 µl sample into the 20 µl volume reactions. Bulk quantities from 10 000 to 100 000 reactions are also available.

Benefits:

  • Robust 4X qPCR mix for high sample volume input up to 10 µl
  • Detects <5 RNA copies per reaction
  • Successfully tested for Sars-CoV-2 detection
  • Reverse transcription and qPCR in one tube
  • Ideal for multiplex reactions

high sensitivity RT qPCR

4X 1Step RT qPCR Probe Kits are designed for sensitive detection of specific RNAs, including virus RNA, in diluted high-volume samples.
They combine a robust 4X qPCR mix (with Hot Start PCR Polymerase) with a 20X blend of thermostable and extremely active Reverse Transcriptase & advanced RNase Inhibitor (RT4 Mix, 20X).
This formulation allows for a high sample input volume with a reliable outcome of a single step RT qPCR when working with low copy number samples below 5 copies per reaction.
4X 1Step RT qPCR Probe Kits ensure the robust performance of both reverse transcription and qPCR reactions, which allows for the highest sensitivity viral RNA detection under fast qPCR cycling conditions.
The performance of the kits has been tested for Sars-CoV-2 detection according to the recommended Charité Berlin protocol with appropriate primers/probes. Check Application Note for Sars-CoV-2 detection.
PCR Water supplied in the kit ensures the best performance and reproducibility of the results.
Depending on your instrument requirements, the kit is available as no ROX, ROX L (low), and ROX H (high) versions.

Order a sample:

More Information:

  1. Application Note for Sars-CoV-2 detection using 4X 1Step RT qPCR Kits
  2. Product Insert example
  3. MSDS
  4. BULK Offers
  5. VIDEO

Thank you!

Your highQu team

Professionally Simple