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4X 1Step Probe RT-qPCR Kits include 4X qPCR mix for the highest sample volume input

4X 1Step Probe RT-qPCR Kits  include 4X qPCR mix for highest sample volume input

Our classical 1Step RT qPCR Probe Kits are very popular products for Sars-CoV-2 detection needs. However, scientists who work with low copy number Sars-CoV-2 RNA samples requested to have 4X concentrated qPCR mixes in these kits instead of 2X qPCR mixes.
4X concentrated qPCR mixes allow for much more sample input. Adding higher sample volumes into RT qPCR reaction may increase the detection sensitivity and highly reduce the number of false negatives in Sars-CoV-2 detection cases.
To meet customers' needs for larger sample input volumes, highQu presents 3 1Step RT qPCR Probe Kits as 4X concentrated versions.

NEW 4X 1Step Probe RT-qPCR Kit versions - developed for more sample input, enabling working with highly diluted low-copy-number samples.
They include a 4X concentrated qPCR master mix, that allows adding up to 10 µl sample into the 20 µl volume reactions. Bulk quantities from 10 000 to 100 000 reactions are also available.


  • Robust 4X qPCR mix for high sample volume input up to 10 µl
  • Detects <5 RNA copies per reaction
  • Successfully tested for Sars-CoV-2 detection
  • Reverse transcription and qPCR in one tube
  • Ideal for multiplex reactions

high sensitivity RT qPCR

4X 1Step RT qPCR Probe Kits are designed for sensitive detection of specific RNAs, including virus RNA, in diluted high-volume samples.
They combine a robust 4X qPCR mix (with Hot Start PCR Polymerase) with a 20X blend of thermostable and extremely active Reverse Transcriptase & advanced RNase Inhibitor (RT4 Mix, 20X).
This formulation allows for a high sample input volume with a reliable outcome of a single step RT qPCR when working with low copy number samples below 5 copies per reaction.
4X 1Step RT qPCR Probe Kits ensure the robust performance of both reverse transcription and qPCR reactions, which allows for the highest sensitivity viral RNA detection under fast qPCR cycling conditions.
The performance of the kits has been tested for Sars-CoV-2 detection according to the recommended Charité Berlin protocol with appropriate primers/probes. Check Application Note for Sars-CoV-2 detection.
PCR Water supplied in the kit ensures the best performance and reproducibility of the results.
Depending on your instrument requirements, the kit is available as no ROX, ROX L (low), and ROX H (high) versions.

Order a sample:

More Information:

  1. Application Note for Sars-CoV-2 detection using 4X 1Step RT qPCR Kits
  2. Product Insert example
  3. MSDS
  4. BULK Offers
  5. VIDEO

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Your highQu team

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