ALLin™ RPH Polymerase

Applications

  • Long PCR (up to 35 kb) with higher fidelity.
  • Amplification of complex (GC rich) templates.
  • Crude sample PCR, multiplex PCR, TA cloning.

Benefits

  • Long (up to 35 kb) high-fidelity (5X higher than Taq) amplification.
  • High yields in standard and fast PCR, on GC rich DNA and crude samples.
  • 5X ALLin™ PCR Buffer contains optimal Mg2+ and dNTPs.

€199.50*

Product sizes
250 units
€199.50*
1250 units
€899.50*

↑ Collapse variants ↑

Selected Catalog #: HLE0101
Product information "ALLin™ RPH Polymerase "
highQu ALLin™ RPH Polymerase (Robust, Proofreading, Hot-start Polymerase) is the versatile engineered enzyme combining best polymerase properties for excellence in most demanding PCR applications, like low copy detection, long or high fidelity PCR, amplification of complex templates, crude sample PCR and multiplexing.
ALLin™ RPH Polymerase has 5 times higher fidelity than Taq DNA Polymerase and produces A-tailed products suitable for ligating into TA cloning vectors.
For the maximum convenience the ALLin™ RPH Mastermix, 2X is available.

Related Products

1Step RT qPCR Probe Kit
highQu 1Step RT qPCR master mixes in combination with a blend of thermostable and extremely active Reverse Transcriptase & advanced RNase Inhibitor (RT Mix) allow for a single step one tube RT qPCR. qPCR master mixes are based on the small molecular inhibitor technology Hot Start PCR allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions. They provide excellent results on both AT/GC rich templates and guaranty rapid extension with early Ct values with minimum or no optimization. Our master mixes are supplied with PCR Water to guaranty the best performance. To suit the broad instrument range the 1Step RT qPCR Probe Mixes are available in three versions – without ROX, with low or high ROX concentration.

€224.50*
ALLin™ Red Taq Mastermix
highQu ALLin™ Taq DNA Polymerase is the versatile engineered enzyme which in combination with the optimized ALLin™ buffer provides higher success rates in demanding PCR applications like amplification of complex templates, crude sample PCR and fast cycling. ALLin™ Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 10^4 (a number of correct nucleotides incorporated before the error occurs) and produces A-tailed products suitable for ligating into TA cloning vectors. The convenience of ALLin™ Taq DNA Polymerase is maximized by the use of 2X Red Mastermix providing the additional advantage of reduced pipetting and minimized errors. ALLin™ Red Taq Mastermix, 2X is premixed with red dye and density reagents for direct loading on the gels after the PCR. In a 2% agarose TAE gel the dye migrates with~350 bp DNA, in 1% agarose TAE gel with ~ 600 bp DNA fragments. The mastermix is even supplied with the PCR Water, and the only thing to add is the template with primers.

€99.50*
ALLin™ Mega HiFi DNA Polymerase
Derived from our HiFi polymerase, the highQu ALLin™ Mega HiFi DNA Polymerase provides much lower error rate PCR with a 100 higher fidelity compared to Taq. The ALLin™ Mega HiFi DNA Polymerase is engineered to be much faster and to generate a higher yield of long PCR products up to 20 kb from complex GC-rich templates. Therefore the ALLin™ Mega HiFi DNA Polymerase is an excellent choice for longer and very complex PCR applications where the highest fidelity is demanded. It is an enzyme of choice for cloning and all kind of sequencing applications including NGS. Generated blunt-ended PCR products are suitable for ligation into blunt vectors.  For maximum convenience, the ALLin™ Mega HiFi Mastermix, 2X (HLM0201) and ALLin™ Mega HiFi Red Mastermix, 2X (HLM0301) are available.

€99.50*
SampleIN™ Direct PCR Kit
SampleIN™ Direct PCR Kit is a premium tool for a fast direct PCR eliminating the need of tedious template purification. The kit is excellent for direct PCR from mouse tail or ear, mammalian tissues, hair follicle, buccal swabs and blood. Rapid 15 min DNA extraction using DPK Lysis and Protease Buffers in a single tube generates PCR template extract which is further amplified under fast cycling conditions with a hot-start Taq master mix that includes red dye for direct gel loading. In a 2% agarose TAE gel the red dye migrates with~350 bp DNA, in 1% agarose TAE gel with ~ 600 bp DNA fragments. The ALLin™ HS Red Taq Mastermix includes a hot start Taq DNA Polymerase what ensures high yield, specific, low background amplification. Mix components allow for a fast PCR cycling and increase success when working with complex templates or multiplexing. Generated A-tailed PCR products are suitable for ligating into TA cloning vectors, sequencing and other applications.

€98.00*
ALLin™ Isothermal DNA Amplification Kit
ALLin™ Isothermal DNA Amplification Kit enables detection of as little as 5 target DNA molecules in a short time of 20 minutes even without the use of a thermal cycler. The Kit includes a 2X master mix with optimized high-performance buffer, dNTPs and a recombinant Bst Polymerase large fragment having strong 5’ - 3’ strand displacement activity and efficient 5’-3’ polymerase activity working at 55-70°C. The Polymerase has neither 5’ - 3’ nor 3’ - 5’ exonuclease activity and retains only minor reverse transcription activity. The kit includes PCR water; only templates and primers have to be supplied by the user. Quantitative Fluorescent Dye, 20X is included for an optional use, for a real-time detection in FAM channel on any qPCR cycler. ALLin™ Isothermal DNA Amplification Kit is a tool of choice for such applications like LAMP, WGA, RAM with an additional advantage of higher temperature reactions, what makes amplification of complex and GC-rich templates more efficient. Technical characteristics of Bst DNA Polymerase large fragment: Strong 5’ - 3’ strand displacement activity 5’-3’ polymerase activity No 5’ - 3’ exonuclease activity No 3’ - 5’ exonuclease (proofreading) activity Minor reverse transcriptase activity (for RNA, use ALLin™ Isothermal 1Step RNA Amplification Kit (#IRK0101) which includes RTase) Optimal amplification temperature is 65°C. Working temperature range is 55-70°C. Optimal reaction time is 20 minutes, if needed, the reaction can be performed 30 to 60 minutes. The enzyme is inactivated in 10 minutes at 80°C. Quantitative Fluorescent Dye has the excitation max. at 482 nm and emission max. at 512 nm. The use of this product in certain applications may be covered by patents. The user has to analyse all applicable Limited Use Label Licenses and may need licensing for certain cases.

Variants from €179.50*
€98.00*
ALLin™ Isothermal 1Step RNA Amplification Kit
ALLin™ Isothermal 1Step RNA Amplification Kit enables detection of < 5 target RNA molecules in a short time of 30 minutes without the use of PCR cycler.  An RT5 Mix, the blend of a thermostable reverse transcriptase and highly efficient RNase Inhibitor is included in the kit to initially synthesize cDNA from RNA templates. The cDNA is amplified by Bst polymerase. Kit includes a 2X master mix with optimized high-performance buffer, dNTPs and a recombinant Bst Polymerase large fragment having strong 5’ - 3’ strand displacement activity and efficient 5’-3’ polymerase activity working at 55-70°C. The Polymerase has neither 5’ - 3’ nor 3’ - 5’ exonuclease activity and retains only minor reverse transcription activity. The kit also includes PCR water; only templates and primers have to be supplied by the user. Quantitative Fluorescent Dye, 20X is included for an optional use, for a real-time detection in FAM channel on any qPCR cycler. ALLin™ Isothermal 1Step RNA Amplification Kit is a tool of choice for isothermal amplification of RNA templates and such applications like RT LAMP, with an additional advantage of higher temperature reactions, what makes amplification of complex and GC rich templates more efficient. Technical characteristics of Bst DNA Polymerase large fragment: Strong 5’ - 3’ strand displacement activity 5’-3’ polymerase activity No 5’ - 3’ exonuclease activity No 3’ - 5’ exonuclease (proofreading) activity Minor reverse transcriptase activity Optimal amplification temperature is 65°C. Working temperature range is 55-70°C. The Bst enzyme is inactivated in 10 minutes at 80°C. RT5 Mix includes thermostable reverse transcriptase (modified MMuLV) working at high temperatures and a thermostable RNase inhibitor protecting template RNA from degradation. Optimal 1Step RT amplification reaction time is 30 minutes, if needed, the reaction can be performed 30 to 60 minutes. Quantitative Fluorescent Dye has the excitation max. at 482 nm and emission max. at 512 nm. The use of this product in certain applications may be covered by patents. The user has to analyse all applicable Limited Use Label Licenses and may need licensing for certain cases.

Variants from €215.50*
€98.00*
phi29 DNA Polymerase
Recombinant phi29 DNA Polymerase is a classical enzyme dedicated for use in common isothermal DNA amplification applications that are carried out at moderate temperature based on a strand displacement activity. The enzyme is supplied with an optimized high-performance buffer. The user has to add dNTPs, template and primers. phi29 DNA Polymerase was discovered and characterized by M. Salas. The Polymerase has strong strand displacement activity and efficient 5’-3’ polymerase activity working at about 25-37°C and synthesizing DNA from minor amounts to enormous yield up to visibly increased the viscosity of the reaction mixture. The enzyme has no 5’-3’ exonuclease activity, but has strong 3’ - 5’ exonuclease (proofreading) activity, and may degrade primers, therefore the use of 3’ protected exo-resistant primers is recommended in the literature. The enzyme can be heat-inactivated, tolerates dUTP and produces blunt-ended DNA. Most important technical characteristics of phi29 DNA Polymerase (based on abundantly available scientific literature): Strong strand displacement activity 5’-3’ polymerase activity on DNA templates (and on RNA templates) Strong 3’ - 5’ (proofreading) exonuclease activity, may degrade unprotected primers No 5’ - 3’ exonuclease activity Optimal reaction temperature is 30°C Working temperature range is 25-37°C, depends on application Optimal reaction time depends on application The enzyme is inactivated in 10 minutes at 65°C. Can extend both DNA and RNA primers Addition of pyrophosphatase may accelerate the reaction The use of this product in certain applications, some additives or protocols may be covered by patents. The user has to analyse all applicable Limited Use Label Licenses and may need licensing for certain cases.

Variants from €159.50*
€98.00*