Our improved 2X 1Step RT qPCR Probe Kits provide highly sensitive target RNA detection when working with a wide variety of samples. The optimized 1Step RT qPCR Pro Mix in combination with a blend of thermostable Reverse Transcriptase and an advanced RNase Inhibitor (RT-Pro Mix) allows for a single step, one tube RT qPCR with great results even in multiplex reactions. The novel RT-Pro Mix ensures safe and efficient RNA template conversion into a single-stranded cDNA. Pure RNA samples, as well as lysed crude samples can serve as templates for one-step RT qPCR. Though the kit was not specifically designed for crude sample qPCR, it has a potential to work well for this application. The robust 1Step RT qPCR Pro Mix is a 2X concentrated qPCR master mix which includes dNTPs, buffer, and the hot start Taq DNA Polymerase. The Hot Start function ensures the highest sensitivity and specificity under both standard and fast qPCR cycling conditions. The kits provide excellent results on both AT and GC-rich templates and show early Ct values with minimum or no optimization. Our kits include PCR Water to guaranty the best performance. To suit the broad instrument range the 2X 1Step RT qPCR Probe Kits are available in three versions – without ROX, with low or high ROX concentration.

SecurRIN™ Advanced RNase Inhibitor is a premium tool for RNA protection from degradation during enzymatic reactions, storage or extraction. It is an extraordinary stable and robust enzyme: it works at up to 60°C temperature, remains active after weeks of room temperature exposure and multiple freezing thawing cycles. It is active in different buffer conditions within a broad pH range of 5.5 to 9.0, and within 0.5 - 1 mM concentration of DTT. SecurRIN™ Advanced RNase Inhibitor is a 50 kDa non-covalent inhibitor of RNase A, RNase B, and RNase C binding them in a 1 to 1 ratio. It is a recombinant protein derived from E. coli strain carrying human placenta RNase Inhibitor gene. SecurRIN™ Advanced RNase Inhibitor does not inhibit RNAses H, 1, T1, T2 and S1 Nuclease. It influences neither the activity nor the performance of DNA polymerases and of Reverse Transcriptases. The enzyme is free from DNAses and RNAses. Ribonuclease Inhibitor is a protein that protects RNA samples from degradation by ribonucleases. Ribonucleases are present everywhere in laboratory environment, they are very robust and ubiquitous enzymes that rapidly degrade RNA by destroying valuable RNA samples during molecular biology or diagnostics workflows. RNase inhibitor binds to RNases and inactivates these enzymes, ensuring the integrity and stability of RNA samples during such processes as RNA isolation, clean-up, cDNA synthesis, RT-qPCR and other applications. A good RNase inhibitor should have high affinity for RNases and strong inhibitory activity under a wide variety of buffer conditions. It shall tolerate different pH values, salt concentrations and shall have high affinity to different types of RNases. It should also be stable under higher temperatures and easy to use in all common RNA-related buffers with minimal interference with downstream applications. Furthermore, it is especially important, that the Inhibitor itself is pure and free from contaminating RNases. highQu offers SecurRIN™ Advanced RNase Inhibitor for RNA protection from degradation during enzymatic reactions, storage or extraction. Is is a 50 kDa non-covalent inhibitor of RNase A, RNase B, and RNase C.  

StainIN™ GREEN Nucleic Acid Stain is a significantly safer alternative to ethidium bromide. It is same easy to use, 4X as sensitive (under Blue LED) and much more secure. More economical as other green dyes, this stain can be also used and disposed with less environmental and health concerns compared to ethidium bromide. It is a fluorescent dye that allows detection of >0,1 ng of DNA in both agarose and polyacrylamide gels. It binds to both dsDNA, ssDNA and RNA and emits green fluorescence when bound to DNA and red fluorescence when bound to RNA detectable under the UV or Blue light and documented with same filters as similar green dyes. StainIN™ GREEN is ideal for DNA extraction from gels for cloning. Smaller than ethidium bromide carcinogenicity has been proved by Ames-test. Mammalian cell mutagenicity tests, both mouse marrow erythrocyte micronucleus and spermatocyte chromosomal aberration tests gave negative mutagenicity results. Carcinogenicity and toxicity of the widely used Ethidium Bromide (EtBr) is one of the biggest safety and environmental problems in the labs where gel electrophoresis is a daily routine. The less toxic nucleic acid dyes address this problem and minimize concerns. The StainIN™ GREEN (SYBR Green-like stains alternative), (SYBR® Green is a registered trade mark of Molecular Probes Inc.) and StainIN™ eco-RED Nucleic Acid Stains by highQu provide a much less-carcinogenic, significantly safer alternative to EtBr while delivering even several times higher detection sensitivity. Same like EtBr, these stains are used during the process of electrophoresis in an agarose gel or PAAG and in the electrophoresis running buffer. Nucleic acid stains are typically divided into two groups – they are either intercalating dyes or minor groove binders. The StainIN™ GREEN and StainIN™ eco-RED Nucleic Acid Stains belong to the group of intercalating dyes that bind to NA and produce the fluorescence signal. Same like many other suppliers of similar products, highQu retains the right to keep the precise chemical information undisclosed and proprietary. The StainIN™ GREEN Nucleic Acid Stain as well as the StainIN™ eco-RED Nucleic Acid Stain are both supplied in water. Detailed safety information is available in MSDS sheets of the stains. Despite proven less mutagenicity, all nucleic acid stains, including SYBR® Green or other shall be handled with care. Used electrophoresis buffer with a stain as well as melted gels shall be run through approved (carbon) filters. If the absence of residual fluorescence is confirmed, the liquids can be disposed with plenty of water down the drain. Alternatively, all used liquids can be autoclaved as the StainIN™ GREEN and StainIN™ eco-RED Nucleic Acid Stains degrade during the long exposure to above 100ºC temperature. The safety office shall be consulted periodically to match local regulations, as they vary and change.    

highQu dNTP sets and mixes meet all highest industry standards and allow for unrivaled performance of your PCR and other DNA synthesis, labeling or sequencing reactions. Produced under the stringent quality monitoring conditions, they guaranty reproducible results. More than 99% HPLC purity eliminates inhibitions of PCR and allows for increased yields with higher dNTP concentrations. Exceptional stability of dNTPs allows for short term ambient temperature shipments, short term room temperature storage or long PCR of more than 30 kb targets, as well as long amplifications exceeding 40 cycles.