Take5™ 100 bp DNA Ladder

Applications

  • DNA sizing on agarose gels.
  • Approximate DNA quantification on agarose gels.

Benefits

  • Room-temperature-stable ladders - always ready to use.
  • Sharp bands, bright reference bands, indicated DNA mass.
  • Supplied with loading dye for DNA samples.

€107.00*

Product sizes
200 applications
€107.00*

↑ Collapse variants ↑

Selected Catalog #: DNL0202
Product information "Take5™ 100 bp DNA Ladder"

highQu ready to use DNA ladders are mixtures of highly purified plasmid digests and PCR products. The high ladder purity allows for exceptional stability and room temperature storage. Ladders are ready to be directly loaded on agarose gels and are supplied with the loading dye solution for probe DNA. They provide sharp bands for DNA sizing and allow for approximate DNA quantification by comparing the amount of DNA of the band similar in size to your sample band.

Proper agarose gel preparation is a crucial step in DNA electrophoresis. Here are some detailed tips to ensure optimal separation and visualization of DNA fragments:

Proper agarose gel preparation is a crucial step in DNA electrophoresis. Here are some detailed tips to ensure optimal separation and visualization of DNA fragments:

Gel casting:

  • Choose the appropriate agarose concentration based on the expected fragment size range.
  • Measure the required amount of agarose and add it to the appropriate volume of buffer.
  • Heat the mixture in a microwave until the agarose is completely dissolved.
  • Allow the mixture to cool to a temperature that is safe to touch but still liquid before pouring it into the gel tray.
  • Avoid creating air bubbles during pouring by slowly and gently filling the gel tray.
  • Wait till the agarose gel is completely formed.
  • Immerse the gel into the tray with the buffer, remove the combs and ensure the gel is completely covered by electrophoresis buffer.

DNA sample loading:

  • Prepare DNA samples by mixing them with loading buffer supplied with the DNA ladder.
  • This buffer helps the samples sink into the wells and provides tracking dyes for visualization.
  • Load a suitable volume of each DNA sample into the wells.
  • Use DNA ladder or molecular weight marker on both sides of the sample or gel for reference.
  • highQu DNA ladders are ready to use and do not require any extra preparation for loading.
  • Mix the ladder and the sample well before loading on the gel.

Electrophoresis running conditions:

  • Set the desired voltage according to the fragment size and the resolution required.
  • Lower voltages are suitable for resolving larger fragments.
  • Start the electrophoresis run and monitor the migration of tracking dyes.
  • Stop the run once the desired separation is achieved, but be cautious not to allow the DNA bands to run off the gel.
  • Stain the gel post electrophoresis according to the conditions described by Nucleic Acid Stain supplier, for example use highQu StainIN™ GREEN NA Stain.

Related Products

2X 1Step RT qPCR Probe Kit
Our improved 2X 1Step RT qPCR Probe Kits provide highly sensitive target RNA detection when working with a wide variety of samples. The optimized 1Step RT qPCR Pro Mix in combination with a blend of thermostable Reverse Transcriptase and an advanced RNase Inhibitor (RT-Pro Mix) allows for a single step, one tube RT qPCR with great results even in multiplex reactions. The novel RT-Pro Mix ensures safe and efficient RNA template conversion into a single-stranded cDNA. Pure RNA samples, as well as lysed crude samples can serve as templates for one-step RT qPCR. Though the kit was not specifically designed for crude sample qPCR, it has a potential to work well for this application. The robust 1Step RT qPCR Pro Mix is a 2X concentrated qPCR master mix which includes dNTPs, buffer, and the hot start Taq DNA Polymerase. The Hot Start function ensures the highest sensitivity and specificity under both standard and fast qPCR cycling conditions. The kits provide excellent results on both AT and GC-rich templates and show early Ct values with minimum or no optimization. Our kits include PCR Water to guaranty the best performance. To suit the broad instrument range the 2X 1Step RT qPCR Probe Kits are available in three versions – without ROX, with low or high ROX concentration.

Variants from €247.00*
€224.50*
25 mM dNTP Mix
highQu dNTP sets and mixes meet all highest industry standards and allow for unrivaled performance of your PCR and other DNA synthesis, labeling or sequencing reactions. Produced under the stringent quality monitoring conditions, they guaranty reproducible results. More than 99% HPLC purity eliminates inhibitions of PCR and allows for increased yields with higher dNTP concentrations. Exceptional stability of dNTPs allows for short term ambient temperature shipments, short term room temperature storage or long PCR of more than 30 kb targets, as well as long amplifications exceeding 40 cycles.

Variants from €219.50*
€199.50*
1Step RT PCR Kit
highQu 1Step RT PCR Kit combines the 20X RT and RNase Inhibitor mix for efficient reverse transcription and the 2X PCR Mastermix for subsequent amplification of cDNA in the same tube. RT2 Mix, 20X is a blend of the engineered MMuLV (stable at 40-55°C allowing for high yields of long transcripts) with an efficient Ribonuclease Inhibitor protecting the template RNA from RNases. The resulting cDNA is then amplified by the 1Step RT PCR Mastermix, 2X. The PCR mastermix contains our proprietary Hot Start Taq DNA Polymerase. The enzyme activity at room temperature is blocked by small molecular inhibitor. Enzyme becomes active only after heating what allows for highly specific and extremely sensitive amplification, no primer dimer formation and no background. In combination with the optimized buffer the enzyme provides higher success rates in demanding PCR applications like amplification of complex or longer templates and fast cycling. Hot Start Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 104 (nucleotides incorporated before the error occurs), and produces A-tailed products suitable for ligating into TA cloning vectors. For the maximum convenience the Kit includes even the PCR Water to set up the reaction, so the only thing you need to take care of is the high quality RNA template.

Variants from €575.00*
€522.50*
Cozy™ Prestained Protein Ladder
highQu ready to use Prestained Protein Ladders are mixtures of highly recombinant proteins with coupled chromophores providing sharp protein bands and bright colors on denaturing polyacrylamide gels. The high ladder purity allows for exceptional stability and room short term temperature storage. Ladders are ready to be directly loaded on gels without any preparation or heating. They provide sharp bands for approximate protein sizing and allow for monitoring of electrophoresis process and Western transfer efficiency.

Variants from €219.50*
€199.50*