StainIN™ RED Nucleic Acid Stain is a significantly safer alternative to ethidium bromide. It is same easy to use, twice as sensitive and much more secure. At least twice as economical as competing products, this novel stain can be also used and disposed with less environmental and health concerns compared to ethidium bromide. StainIN™ RED is a fluorescent dye that allows detection of >0,3 ng of DNA in both agarose and polyacrylamide gels. It binds to both ds DNA, ssDNA and RNA and emits red fluorescence detectable under the UV light and documented with same filters as ethidium bromide. For cloning applications, UV exposure shall be minimized. Much smaller than ethidium bromide carcinogenicity of the dye has been proved by Ames-test. Mammalian cell mutagenicity tests, both mouse marrow erythrocyte micronucleus and spermatocyte chromosomal aberration tests gave negative mutagenicity results.

highQu qPCR master mixes are based on the small molecular inhibitor technology Hot Start PCR allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions. They provide excellent results on both AT and GC rich templates, in multiplexing and guaranty rapid extension with early Ct values with minimum or no optimization. Our master mixes are supplied with PCR Water to guaranty the best performance. To suit the broad instrument range the ORA™ qPCR Probe Master mixes are available in three versions – without ROX, with low or high ROX concentration.

4X 1Step RT qPCR Probe Kits are designed for sensitive detection of specific RNAs, including virus RNA, in diluted high-volume samples. They combine a robust 4X qPCR mix (with Hot Start PCR Polymerase) with a 20X blend of thermostable and extremely active Reverse Transcriptase & advanced RNase Inhibitor (RT4 Mix). This formulation allows for a high sample input volume with a reliable outcome of a single step RT qPCR when working with low copy number samples below 5 copies per reaction. 4X 1Step RT qPCR Probe Kits ensure the robust performance of both reverse transcription and qPCR reactions, which allows for the highest sensitivity viral RNA detection under fast qPCR cycling conditions. The performance of the kits has been tested for Sars-CoV-2 detection according to the recommended Charité Berlin protocol with appropriate primers/probes. PCR Water supplied in the kit ensures the best performance and reproducibility of the results. Depending on your instrument requirements, the kit is available as no ROX, ROX L (low) and ROX H (high) versions.

highQu ready to use DNA ladders are mixtures of highly purified plasmid digests and PCR products. The high ladder purity allows for exceptional stability and room temperature storage. Ladders are ready to be directly loaded on agarose gels and are supplied with the loading dye solution for probe DNA. They provide sharp bands for DNA sizing and allow for approximate DNA quantification by comparing the amount of DNA of the band similar in size to your sample band. The most important factors influencing agarose gel electrophoresis results:  Use the right percentage of the agarose gel for the given size of the DNA to be analysed. Prepare the gel according to the instructions of the agarose manufacturer. Use the most sensitive nucleic acid stain (StainIN™ Green) in both agarose gel and in the buffer. Use the same loading dye both for the samples and for the DNA ladder. Select DNA ladder which is supplied with the loading dye solution for the DNA samples (Take5™ 1 kb DNA Ladder). For more precise sizing of the DNA, load the DNA ladder into the two slots of the agarose gel Use enough of the electrophoresis buffer to keep the agarose gel well-covered during the electrophoresis run.  Visualize the agarose gel to compare your sample bands with the ones of the DNA ladder using an optimal gel documentation system.